Ultra-sauri ruwa chromatographyProminenece UFLC

Ana amfani da nazarin sauri a matsayin muhimmin batun fasaha, amma tare da saurin shiga LCMS, haɓaka ƙimar farashi ya zama matsala mai gaggawa, wanda ya zama muhimmin abu don haɓaka sauri.
Bincike mai sauri shine rage lokacin bincike, sau da yawa ta hanyar rage ginshiƙin chromatography ko haɓaka kwararar yanayin kwararar. Duk da haka, ta amfani da masana'antar cikawa mai girman ƙwayoyin 5μm da aka saba amfani da ita, aikin raba ta amfani da kowane hanyar zai ragu sosai, don haka sauri ya rasa ma'ana.
Saboda haka, an haɓaka hanyoyi biyu masu zuwa:
• Amfani da ƙananan particle size fillings
Ta wannan hanyar zai sa rabuwa mai sauri ya yiwu. Duk da haka, saboda karuwar juriya ta kwarara a cikin ginshiƙin chromatography, matsin lamba da ke fuskantar kowane ɓangare na kayan aiki da ginshiƙin chromatography zai ƙaru sosai.
• High zafi rabuwa
Babban zafin jiki yana hanzarta yaduwar abubuwa, yayin da yake rage juriya ta kwarara a cikin ginshiƙin chromatography, kuma yana sa rabuwa mai sauri ya yiwu. Binciken zafin jiki yana da inganci wajen rage matsin lamba na ginshiƙi, amma zafin jiki yana haifar da lalacewar ginshiƙin chromatography, haifar da lalacewar samfurin da sauransu a aikace-aikacen yana buƙatar kulawa.
Don magance matsalar matsin lamba, an haɓaka kayan aiki na musamman don inganta ƙarfin ɗaukar matsin lamba, irin waɗannan kayan aiki yawanci suna da tsada. Duk da haka, wadannan kayan aiki na musamman dole ne su yi sulhu dangane da aikin samfurin da ƙwarewa. Bugu da ƙari, cika ginshiƙin cika da aka yi amfani da shi sosai, yana buƙatar kayan aiki sosai, amfani da hanyar bincike da aka haɓaka ta wannan ginshiƙin chromatography yana sadaukar da universality. Saboda haka, idan aka yi amfani da irin wannan kayan aiki a cikin nazarin HPLC na yau da kullun, ingancinsa zai zama ƙasa.
Prominence tallafawa sauri rabuwa
Prominence yana amfani da daidaitattun sassa waɗanda za su iya biyan bukatun binciken gargajiya da binciken sauri na masu bincike a lokaci guda, kuma za su iya tallafawa zafin jiki na aiki har zuwa 85 ° C don yin nazarin sauri a ƙarƙashin matsin lamba, yayin da aikin bincike da matakan kwararar da kayan aikin ke nunawa ba su da yawa ko da a kan daidaitattun kayan aiki masu tsada.
Prominence UFLC yana tallafawa nazarin sauri ta hanyar fasali masu zuwa:
• Ƙananan madaidaicin bututu da kuma karamin girman kwarara tafkin rage sakamakon girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman girman
• Shim-pack XR-ODS ta amfani da 2.2μm particle size chromatography shafi.
Shim-pack XR-ODS shine sabon ƙarni na masu saurin bincike da ke samun daidaito daga dangantakar ingancin rabuwa da matsin lamba tare da la'akari da ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙaranc

Chromatographic conditions; column: described above, mobile phase: water/acetonitrile (3/7,v/v), flow rate: described above, temperature: 40 ℃, detection: absorbance at 245 nm, sample volume: 4μL(XR-ODS), 10μL(VP-ODS).
Peaks; 1: actophenone, 2: propiophenone, 3: butyrophenone, 4: balenophenone, 5: hexanophenone, 6: heptanophenone, 7: octanophenone.
• Mai samfurin atomatik na SIL-20A yana samun samfurin 10μl mai sauri na dakika 10.

Chromatographic conditions; column: Shim-pack XR-ODS(3mm i.d.×30 mm, 2.2μm), mobile phase: water/acetonitrile(4/6 to 2/8 in 0.4 min, convex gradient), flow rate: 3mL/min, temperature: 80 ℃, detection: absorbance at 245 nm, sample volume: 4μL(each 800 nm ol),.0.1 min delayed injection.
Peaks; 1: actophenone, 2: propiophenone, 3: butyrophenone, 4: balenophenone, 5: hexanophenone, 6: heptanophenone, 7: octanophenone.
• Goyon bayan high zafi bincike har zuwa 85 ° C.
Temperature da sauri bincike: Tare da madaidaicin zafin jiki na ginshiki ya tashi, adadin hasumiya na ka'ida ya karu, ingancin ginshiki ya karu, matsin lamba na ginshiki ya ragu.

Chromatographic conditions;
mobile phase:　water/acetonitrile (3/7, v/v),
detection: absorbance at 245 nm.
Cikakken: 1: Phenylacetone 2: Phenylacetone 3: Phenylbutane 4: Phenylpentone 5: Phenylhexalone 6: Phenylacetone 7: Benzinolone
Tasirin zafin jiki a kan mahada: Babban binciken zafin jiki yana da inganci wajen rage matsin lamba na ginshiƙi, amma babban zafin jiki yana haifar da lalacewar ginshiƙin chromatography, haifar da lalacewar samfurin da sauransu a aikace-aikace yana buƙatar kulawa. Ana amfani da binciken zafi kawai a matsayin taimako don nazarin sauri.
• Mai ganowa tare da babban samfurin mita don samun saurin samun bayanai ba tare da rasa bayanai ba.
• Musamman chromatography hanyoyin canza software, sa yau da kullun chromatography yanayin canza zuwa UFLC yanayin da sauki da kuma tabbatar da m hanyoyin bincike.

Rapid bincike repeatability
Samun maimaitawa na lokacin riƙewar kayan aiki a cikin rarrabuwa mai sauri ya fi wuya fiye da bincike na yau da kullun. The sake dubawa na lokacin kiyayewa (RSD) da kuma murabba'in tushen lokaci ya karu, don haka yana buƙatar ƙarin tsananin aikin samar da ruwa fiye da bincike na yau da kullun. Prominence UFLC zai iya tabbatar da ingantaccen damar samar da ruwa tare da ƙudurin samar da ruwa na 3nL / min, sarrafa amsa na 0.1 seconds lokacin samar da ruwa na gradient, wanda zai iya wasa mafi kyawun sake dubawa na lokacin riƙewa.

Menene High Flow
Manufar Fast Liquid Chromatography ita ce babban gudanarwa, watau ana bincika ƙarin samfurori kowace rana ko kowace awa. Don cimma babban kwarara, ba kawai za a rage lokacin nazarin samfurin guda ɗaya ba, har ma dole ne a inganta duk lokacin zagaye da nazarin samfurin. Manufar neman sauri ba wai kawai rage lokacin bincike ba, har ma yana rage zagayen bincike. Tsarin allurar samfurin Prominence UFLC yana da tsawon dakika 10, wanda ya sa zagaye na bincike ya rage sosai.

Misali na aikace-aikace

Binciken UFLC na amino acids na PTC

Akwai hanyoyi guda biyu na binciken binciken amino acid, daya shine hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta hanyar ganewa ta han Daga hangen nesa na nazari mai sauri, hanyar kawar da ginshiƙi tana da inganci. A nan, an yi nazarin amino acid a cikin gajeren lokaci ta amfani da Shim-pack XR-0DS.
Ana amfani da isocyanate phenyl ester don derivation. Ana ɗaukar sa'a 1 lokacin hanyar post-column derivative, kuma ana iya rage binciken mintuna 25 lokacin amfani da hanyar pre-column derivative ta hanyar chromatography ta yau da kullun zuwa mintuna 4.

Binciken UFLC na pigments

Ana ƙara launuka a cikin abinci tare da kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin Amma lokacin da mahara pigment haɗuwa sa, lokaci guda nazarin daukar dogon lokaci. Wannan misali ne na binciken lokaci guda na pigment da aka yi amfani da Shim-pack XR-ODS Super Fast LC. Sakamakon binciken lokaci guda na 12 nau'ikan launuka kamar yadda ke ƙasa chromatography. Lokacin bincike da ake buƙata don yin amfani da LC na yau da kullun shine mintuna 50, yayin da aka yi amfani da LC mai sauri, binciken ya ƙare a cikin mintuna 6.

Binciken UFLC na polycyclic aromatic hydrocarbons (PAHs)

PAHs sunadarai ne da ke tantance ingancin gurɓataccen yanayi kamar motoci. PAHs suna da ƙarfin cutar kansa, don haka sa ido kan yanayi na waɗannan abubuwa muhimmin ma'auni ne. PAHs suna nuna ƙarfin fluorescence wanda za a iya amfani da na'urorin ganowa na fluorescence don bincike mai mahimmanci.
Don binciken waɗannan nau'ikan PAHs 10, UFLC ya sami bincike mai sauri sau 10 (lokacin bincike na mintuna 5) idan aka kwatanta da LC na yau da kullun (lokacin bincike na mintuna 50) ba tare da yanayin rabuwa ba.