TIRF、 mayar da hankali,FRET、 Hasken haske da fasahar allura ta microscopy sun taimaka wa masana kimiyya su shawo kan matsaloli da yawa a cikin hoton ƙwayoyin halitta. Ti ne zuciyar dukan fasahar, tare da wannan sabon nau'in mai ƙarfi mai juyawa, za ka iya yin amfani da shi a Nikon CFI60 ® Sauƙin amfani da fasahohin da aka ambata a sama tare da taimakon tsarin gani. Jerin Ti yana da samfuran uku, ingantaccen saurin tsarin, haɓaka sassauci da ingantaccen fasali mai yawa ya sa Ti ya zama tsarin da ya dace don bincike na ƙarshe da hoton ƙwayoyin halitta.

Manyan masu zane-zane na Nikon na duniya sun haɓaka rukunin bambanci na waje na musamman. Ta amfani da wannan tsarin da aka haɗa zoben bambanci a cikin jikin microscope maimakon abubuwa, masu amfani ba dole ne su yi amfani da abubuwan bambanci na musamman don kallon hotunan bambanci ba, kuma za su iya samun hotuna masu inganci ta hanyar abubuwan bambanci masu yawa. Hakanan amfani da abubuwa ba tare da bambanci zoben za a iya samunFluorescent image na "cikakken haske".

Tsarin hanyar haske na bambancin bambancin bambancin da aka sanya a cikin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin bambancin Dangane da abubuwan da aka yi amfani da, akwai nau'ikan daban-daban daban guda huɗu don zaɓar (Ti-E / U / S na duniya).

Amfani da Nikon ta high-yi abubuwa, ciki har da60x da 100xTIRF abubuwa, tare da mafi girman lambar 1.49 a duniya, da haɗuwa da zoben gyaran bambanci, don samun hoton bambanci mai ƙuduri wanda ba za a iya kwatanta shi da sauran abubuwan bambanci na yau da kullun ba.

Samun ta amfani da wannan abu"Cikakken haske" hoton fluorescent Saboda hasken haske wanda babu bambancin zobe, a cikin tsarin guda, ba kawai za a iya lura da bambancin ba, har ma za a iya samun hotuna masu haske na "cikakken haske", hotuna masu mayar da hankali, da hotuna na TIRF.

Ta hanyar na'urorin bambanci na waje, za a iya samun hoton bambanci mai haske da ƙuduri har ma da amfani da ruwan ruwa.

Saboda bambancin image daTIRF lura, DIC lura za a iya amfani da iri ɗaya abubuwa, samun hoton za a iya amfani da high daidaito data sarrafawa da kuma image nazarin, misali TIRF hoton kwayoyin halitta profile ma'anar.

Tare da hagu tashar jiragen ruwa, dama tashar jiragen ruwa da kuma kasa* Tsarin tashar jiragen ruwa mai yawa na tashar jiragen ruwa zai iya haɗa kyamara daya a kowane tashar jiragen ruwa. Bugu da ƙari, tsarin sararin samaniya na layered yana iya ƙara tashar jirgin ruwa ta baya, waɗannan halaye suna sauƙaƙe masu amfani da akwatin matattarar launi mai layi biyu da kyamarori da yawa don samun hoto. * Ti-E / B da Ti-U / B Combined zaɓi ƙasa tashar jiragen ruwa

An faɗaɗa damar samun hoto ta amfani da ƙirar tashar jirgin ruwa ta zaɓi. Amfani tare da tashar jiragen ruwa ta gefe yana ba da damar samun hoton tashar biyu tare da kyamarori biyu. Misali a lokacin daFRET (Foster resonance makamashi canja wuri) yana da kula tsakanin fluorescent furotin, da kuma CFP da YFP karfi bambanci sosai, za a iya samun high siginar amo rabo hoto ta hanyar daidaita daya kamara hankali kwatanta.

Ti ta yi amfani da layered tsari da cikakken amfani da Unlimited nesa optical tsarin, kuma zaiPFS hadewa zuwa abu mai juyawa. Za a iya gabatar da abubuwa biyu na zaɓi ban da PFS a cikin hanyar haske ta hanyar pad high block, tare da tsarin za a iya amfani da laser pins, na'urorin kunnawa na haske da na'urorin fluorescence na sauka a lokaci guda. Kowane layer na lantarki fluorescent tace launi block akwatin za a iya sarrafa daban.

Ta hanyar gabatarwaA 870nm wavelength block na'urar, masu bincike za su iya amfani da kusan infrared fluorescent dyes ciki har da Cy5.5. An haɓaka halayen gani daga ultraviolet zuwa infrared, yawan abubuwan da ke akwai yana ƙaruwa, yana ba da damar mai da hankali a cikin aikace-aikace masu yawa, ko a cikin Ca na ultraviolet²⁺Matsayi auna ko infrared kewayon laser pins.

Aika96 rami allon yi uku tashoshi (biyu tashoshi fluorescence da bambanci) da sauri harbi, da sauri fiye da sau biyu.

Mai mayar da hankali yana da babban shinge ga lura da jerin lokaci. NikonTsarin PFS yana gyara mai mayar da hankali da zai iya faruwa a lokacin dogon lokacin lura da kuma lokacin da aka saka. Za a iya kiyaye mayar da hankali ko da yana amfani da abubuwan da ke da ƙarfi ko fasahohi kamar TIRF. Bugu da ƙari, haɗawa da PFS a kan mai juyawa na abu yana taimakawa wajen adana sarari kuma ba ya iyakance tsarin layered mai girma na Ti. PFS yana amfani da ingantaccen tsarin diyya na gani don daidaitawa a ainihin lokacin Z axis. Lokacin da ba a buƙatar amfani da PFS ba, za a iya cire shi daga hanyar haske.

Cibiyar sarrafawa ta dijital da Nikon ta haɓaka ta hanyar rage lokacin sadarwa tsakanin sassa, haɓaka saurin kayan haɗi daban-daban, wanda ke haɓaka saurin aiki gaba ɗaya sosai.PC sarrafawa inganta lantarki sassa na Ti, rage amsa lokaci tsakanin aiki umarnin zuwa motsi, don haka yi babban gudun sarrafawa a gaba ɗaya. Ta hanyar ƙara firmware mai hankali, jimlar lokacin aiki na sassan lantarki ya rage sosai, misali, jimlar lokacin da ake buƙata don samun hoto mai ci gaba da tashoshi uku (fluorescence mai tashoshi biyu da bambanci) ya rage sosai, yana rage gubar haske ga ƙwayoyin halitta.

Synchronous sarrafa da dama lantarki sassa, kamar abubuwa mai juyawa, fluorescent tace launi block, haske lattice, focuser mai juyawa, da kuma kaya tebur, masu bincike iya gudanar da multi-dimensional lantarki gwaji. Saurin motsi na kayan haɗi da samun hoto yana rage lokacin fallasa gaba ɗaya, rage hasken haske mai dacewa, yana taimaka wa masu bincike su sami ƙarin ma'ana.

Operations da kuma/ ko canza abubuwa, tace block, XY carrier tebur, motsawa / toshe tace gudun karuwa sosai, da masu bincike iya mayar da hankali kan lura da kuma image samun. Sabon mai kula da aka haɓaka zai iya yin rikodin da kwafin yanayin lura, yana amfani da linzamin kwamfuta don sarrafa tashar ɗaukar kaya, kuma dukan microscope yana kama da ɓangarorin ido da hannun mai bincike.

Fasahar gani da Nikon ta inganta tana ba da hanyoyi da yawa don lura da samfurin, yana nuna wa masu bincike kowane cikakken bayani game da ƙwayoyin.

Nomarskibambancin tsoma baki (DIC)

Daidaitaccen bambanci mai girma da ƙuduri mai girma yana da mahimmanci don lura da microstructures. Nikon na musammanTsarin DIC yana samun hotuna masu ƙuduri har ma a ƙananan ƙarancin ƙarancin. Sabon DIC slider (bushewa) yana ba da zaɓuɓɓuka biyu na babban ƙuduri da babban bambanci. Ana iya sanya DIC daukar hoto a cikin akwatin tacewa na lantarki, yana rage lokacin canzawa na DIC da kuma lura da fluorescence.

Za a iya amfani da bambanci image lokacin da luraCFI Plan Fluor ADH 100x (Oil)。 Wannan abu yana rage halo na bambancin hoto idan aka kwatanta da abubuwan bambancin gargajiya, yana haɓaka bambancin hoto.

Amfani da HighNa'urar mayar da hankali na NA na iya yin lura da duhu. Ana iya lura da microparticles na dogon lokaci kuma kauce wa haske whitening.

HMC abubuwa daHMC focusing kayan haɗin za a iya samun high bambanci, halo-free hoto kamar 3D, wanda za a iya amfani da shi don noma m samfurin a cikin filastik Petri kwanoni.

CFI S Shirin Fluor ELWD/ELWDbambancin abubuwa

New R & D abubuwa ga kusan UV (Ca2 +) zuwa kusan infrared wavelength kewayon yana da babban m, kuma inganta launi bambancin gyara. Ana iya samun hotuna masu inganci a yanayin haske daban-daban.

Shirya Apochromat 20xAbubuwan

sabon20x abubuwan da aka haɗa da Nikon mallakar VC abubuwan da aka gyara da bambancin launi na axial zuwa 405nm, shine kyakkyawan abubuwan da aka yi amfani da su don lura da ko'ina da fasahar kunnawa ta haske.

Duk maɓallin maɓallin da sarrafawa masu canzawa don aikin lantarki suna da ƙirar mutum sosai, kuma masu bincike za su iya mai da hankali kan bincike ba tare da tasirin aikin microscope ba.

canzawa na fluorescent tace block, abu canzawa,Z axis thickness / daidaitawa, PFS bude / kashe iko, watsa haske bude / kashe iko duk za a iya sauya da sauri ta hanyar maɓallin da ke cikin jikin microscope.

Ana iya sarrafa high-gudun lantarki ta hanyar hannu ko mutum-inji mai sarrafawaXY daukar kaya tebur da Z axis.

microscope yanayin ciki har da abu bayanai daZa a nuna yanayin buɗewa / kashewa na PFS akan allon VFD. Tune.

PFSCompensation ayyuka

PFS diyya aiki ne mai sauki don sarrafawa, kawai daya maɓallin zai iya canzawa m/ daidaitawa.

Za a iya aiki da microscope ta hanyar nesa iko panel da kuma tabbatar da halin yanzu na microscope. Hakanan ana iya canzawa yanayin lura ta atomatik ta hanyar maɓallin tsoho. Sauyawa daga bambanci zuwa lura da fluorescence za a iya kammala da maɓallin guda ɗaya.

Yana karkata gaban jikin microscope ɗan kadan baya, yana rage nesa tsakanin idanun mai aiki da samfurin kimanin40mm， Inganta operability.